THE LOST CHROMATOGRAPHY LESSON

 

 

“Let me take the glass off?”

 

Background Science Summary:

 

The wonder of the chromatography experiment, whether on earth or in the zero-G heavens,  is the capillary action of water movement in the filter paper strip.  Defying both the force of gravity and the expected inertia of the water drop, the liquid advances toward the ink spot.   Were it not for an understanding of capillary action, the gradual soaking toward the ink spot would be a mystery. Indeed, what is the process which separates various colors of ink from the ink spot?      

 

How it works:  Though ink appears to be composed of one color, it is not.  Actually, several colored pigments blend together to make the color.  This is known as a mixture.  Artists mix different paint colors, like red, blue and green, to achieve a desired color which is a combination of the three basic colors.   Because of  chromatography, ink becomes  soaked in the water drop so that the different pigments begin to separate or  "bleed" apart.  This results in the true colors being discovered.   For Christa, one of the true colors in the black ink spot separated out by chromatography was blue.     

 

But more discussion of the science is needed.  Why does one ink separate apart from another, and why does the water move toward the ink spot in the filter paper strip? The first question’s answer is:  When a substance dissolves in a liquid like water, it is said to be soluble.  Since this quality of being soluble differs in substances, each color will dissolve at a different rate so ink color separation occurs as the water soaks into the black ink spot.   While that explains the separation, what about the creeping movement of the water toward the ink spot?                                                                                          

 

One  definition of capillary action is:  The action (some call it wicking) whereby a liquid like water spontaneously slowly moves up thin tubes and/or  fibers as a result of forces, adhesive and cohesive, as well as surface tension.  While this is a definition of the phenomena, what actually causes it?  The answer is altogether complicated such that scientists have studied the process for centuries.  Yet, without it mankind would be in severe difficulties for survival.  Plants grow as a result of the process of capillary action providing food for life.  Suffice it to say that capillary action consists of having various forces contributing to the adhesion of water or another liquid to a solid such as  wood as well as the  cohesion of water or other liquid molecules with one another playing  a critical role in the climbing or moving process.

 

 

Christa Planning Chromatography  in Space Demonstration

Click on

chromatography_ground_practice.wmv (8.1 MB)

to play the video.

 

In the above video, Christa deals with planning the application of the water via the dropper to the chromatography test paper.  She considers camera angles in order to record the capillary/osmosis process in zero-G as the water  ascends toward the ink spot.   The team discusses how launch and on-orbit operations may affect the stowed test paper strip prior to performing the experiment.

 

The photo below  is cropped from Christa’s  ground exercise of the chromatography experiment video. 

 

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Classroom Version of  Chromatography Lost Lesson

 

The following demonstration closely replicates Christa’s experiment seen in the above video:

Background:

 

A simple technique is used to separate colored ink pigments  from black ink. Being soluble, the ink will dissolve  in water and the mixture or  solution will then be absorbed on filter paper. As the dissolved solvent rises on the paper and evaporates, it leaves a trail of  colors on its path.

 

Materials:

 

1. Ordinary filter paper or paper towel (about a half inch by six inches)
2. Black felt-tip pens
3. Test tube(s)
4. Test tube holder (rack)
5. Paper clip(s)
6. Cork(s) which fit the mouth of the test tube(s)

 

Process:

 

* Cut the  filter paper into a strip so that fits within the  test tube.  Next, cut the lower end of the paper strip so that it points toward the bottom of the test tube.   (see drawing above right)  Draw a light pencil line across the paper strip where the point begins.   Bend open  a paper clip and stick one end into the cork test tube stopper.  Slide the paper strip into the clip in order to hang the strip into the test tube. Watch the video of Christa to see how her apparatus was constructed.   Note in the video that the sides of the strip should not touch the glass.

*Just as Christa’s filter strip has a black ink spot with a black felt-tip pen, likewise, place a small ink dot on the center of the pencil line.

*Rather than applying a drop of water with a dropper as did Christa, add enough water to the test tube to cover the bottom of the filter paper but not enough to reach the ink dot.  (Note the video audio commentary by Christa about where the water should be applied to the strip of filter paper.)

*Next, place the filter paper in the test tube with the dot above the water level just as Christa did in the video. 

*Watch what happens compared to what is seen in Christa’s video. 

*While the video shows the water only barely going up the filter paper above the dot, for this experiment let the  water go up the filter paper within an inch of the top, then remove the strip and let it dry.

 

*Are there color spots at different lengths above the original start point?   Try the experiment again with a different pen.  What do you see?

 

Analysis:

 

Because the ink was water-soluble, it dissolved  into the filter paper. As the ink moved up because of capillary action on the paper, the various pigment colors were deposited  based both on their solubility in water as well as  their attraction to the paper.  It is seen that the more soluble the ink color, the higher the molecules in the pigment ascended up the paper.

 

Questions to Answer:

 

1.  What color(s) separated from Christa’s black ink?   What color(s) separated from the above experiment?  Explain the difference in your own words.

2.  How might the separation of ink colors be used to solve a crime?

 

What Would Have Happened on Challenger?

 

                This question is best answered by actually performing the    above experiment.  In the process, ask these questions:

 

1. How would the absence of gravity have affected the apparatus designed for the classroom?
2. Would the experiment have been more difficult to perform without gravity?  Why or why not?
3. Could Christa have performed the experiment in orbit as it was in the classroom, i.e., with the water in the bottom of the test tube?   Why do you think Christa’s experiment used an eye dropper to apply the water solution?
4. Could the chromatography experiment have been combined with the lost hydroponics lesson apparatus for the on-orbit demonstration by Christa?  Why or why not?  How might you have designed an added chamber for  the chromatography demonstration?

Back to the Table of Contents

 

For added information or copies of the project, contact the project editor Jerry Woodfill, at ER7, NASA JSC, Houston , TX 77058 .  Phone: 281-483-6331,  E-mail: jared.woodfill-1@nasa.gov

 

The project is a work of the Automation, Robotics, and Simulation Division of the NASA Johnson Space Center , Houston , Texas . As part of the Space Educators’ Handbook, its ID identifier  is OMB/NASA Report #S677.